MS1 chromatogram data missing for some peptides in PRM on an orbitrap

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MS1 chromatogram data missing for some peptides in PRM on an orbitrap julian matytchak  2025-07-09 01:21
 

Dear Skyline support team,

We are trying to establish a PRM method on a Thermo Fusion Lumos (orbitrap) instrument using the "PRM on an orbitrap" tutorial.

When viewing our PRM data in Skyline, we noticed that the precursor ion chromatogram (MS1) data is missing for several of our target peptides.
An example of this can be seen in the attached picture, showing three different precursor ions of the same peptide. Only for the first precursor, MS1 data is displayed.

It is probably just a matter of settings, but I don't entirely understand why the data is displayed for some precursors and not for others.

Thank you in advance,

Julian