import a peptide list with RT

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import a peptide list with RT lesurant  2025-11-17 06:07
 

Hello,

I would like to quantify peptides at the MS1 level in Skyline using identifications that were generated by an external tool (PNovo3).

Is there a way to import a peptide list with associated retention times without going through the iRT predictor workflow? The iRT module read the retention times already be present in the Skyline document, which require peak picking beforehand, something I cannot reasonably do manually.

Thank you for your help.

 
 
Brian Pratt responded:  2025-11-17 11:09

This discussion will probably set you up:
https://skyline.ms/__r3/announcements-thread.view?rowId=37483

Best Regards,

Brian Pratt