Dear Skyline team,
I am a user of Skyline for small-molecule analysis.
I wanted to quantify native analyte (small molecule) concentration in blood-based samples using labeled standards (based upon absolute quantitation). I usually import the transition list with native-labeled transition pairs for the targeted small-molecule list, with light-heavy combination. However, an error occur all the time related to document setting i.e., saying "the isotope modification type heavy does not exist in the documents settings" before error checks, while identify column for the option "Label type". Thus, it stops importing the transition list. But, I always set internal standard types "heavy" in the molecule settings. On the other hand, there is no error occur when ignoring this column, hence the transition list gets imported. Can you please suggest, which document settings need to be changed or modified? as I checked all the options but couldn't figured it out. So, that I can quantify absolute native concentrations in the samples using the calibration curve generated with labeled standards using a document grid in Skyline.
I am attaching a transition list I use in .xlsx file and screenshots of the errors in a .pptx file for your check.
Thank you.
Kind regards,
Kapil