Hello!

My lab made a standard curve mixing 4 different Ceramide Standards with the concentrations 100µM - 75µM - 50µM - 25µM - blank.
So I have 5 samples that were measured with an Obritrap, one sample called 100µM with 100µM of all of the 4 Ceramide Standards and the same for the rest.

I also have 6 biological samples that need to be quantified for Ceramides. They do not contain the standards.

I have been tasked with making a calibration curve to quantify the ceramides in the 6 biological samples.
Everything worked well with the transition list, importing results and setting up the calibration curve. The R-squared and calculated concentrations look fine but I worry that this method is not correct as the standards are not present in the biological samples but Skyline pretends that they are.

But when I add the concentrations of the ceramides to the Document Grid it fills in those concentrations for every molecule. And I cannot choose which calibration curve I want to use in the Document Grid.

I either need a "Surrogate Calibration Curve" / to choose with Calibration Curve to use or to choose from which molecules and samples the calibration curve should be created.
(Issue 964 Feature Request might work but I cannot find this in Skyline)

I use Skyline daily with the Molecule Interface.

Sorry for the long text